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megashortscript t7 rna polymerase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher megashortscript t7 rna polymerase kit
    Megashortscript T7 Rna Polymerase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/t7+rna+polymerase+megashortscript+kit/pmc01142345-81-17-22
    Average 90 stars, based on 1 article reviews
    megashortscript t7 rna polymerase kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Generated:

    Article Title: Altered Strand Transfer Activity of a Multi-drug-resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant with a Dipeptide Fingers Domain Insertion
    Article Snippet: T7 RNA polymerase Megashortscript kit (Ambion) was implemented for synthesis of Donor 80 nucleotide (nt) RNA using the purified PCR product as a template and the primer PCIS 14 (TGGTAAACATTCTTGAGTGC). .. The 119 nt acceptor RNA template were generated from T7 RNA polymerase Megashortscript kit (Ambion) using the previously described plasmid pAM 2 30 . ..

    Plasmid Preparation:

    Article Title: Altered Strand Transfer Activity of a Multi-drug-resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant with a Dipeptide Fingers Domain Insertion
    Article Snippet: T7 RNA polymerase Megashortscript kit (Ambion) was implemented for synthesis of Donor 80 nucleotide (nt) RNA using the purified PCR product as a template and the primer PCIS 14 (TGGTAAACATTCTTGAGTGC). .. The 119 nt acceptor RNA template were generated from T7 RNA polymerase Megashortscript kit (Ambion) using the previously described plasmid pAM 2 30 . ..

    Article Title: The newly discovered Q motif of DEAD-box RNA helicases regulates RNA-binding and helicase activity
    Article Snippet: .. A 44-nucleotide-long RNA was transcribed off the Hin d-III-cut plasmid using the T7 RNA polymerase MEGAshortscript kit (Ambion). ..

    Purification:

    Article Title: Structural analysis reveals the characteristic features of Mtr4, a DExH helicase involved in nuclear RNA processing and surveillance
    Article Snippet: The coding sequence for S. cerevisiae tRNA iMet preceded by a T7 promoter sequence was cloned in a pUC19 vector by assembly of overlapping phosphorylated oligonucleotides. .. RNA was transcribed using the T7 RNA polymerase MEGAshortscript kit (Ambion) in the presence of [α-32P]UTP and purified on a 10% (wt/vol) polyacrylamide gel containing 7 M Urea. ..

    Article Title: Altered Strand Transfer Activity of a Multi-drug-resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant with a Dipeptide Fingers Domain Insertion
    Article Snippet: The PCR product was then purified by agarose gel electrophoresis and Qiaquick gel extraction kit (Qiagen). .. T7 RNA polymerase Megashortscript kit (Ambion) was implemented for synthesis of Donor 80 nucleotide (nt) RNA using the purified PCR product as a template and the primer PCIS 14 (TGGTAAACATTCTTGAGTGC). .. The 119 nt acceptor RNA template were generated from T7 RNA polymerase Megashortscript kit (Ambion) using the previously described plasmid pAM 2 30 .

    Polymerase Chain Reaction:

    Article Title: Altered Strand Transfer Activity of a Multi-drug-resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant with a Dipeptide Fingers Domain Insertion
    Article Snippet: The PCR product was then purified by agarose gel electrophoresis and Qiaquick gel extraction kit (Qiagen). .. T7 RNA polymerase Megashortscript kit (Ambion) was implemented for synthesis of Donor 80 nucleotide (nt) RNA using the purified PCR product as a template and the primer PCIS 14 (TGGTAAACATTCTTGAGTGC). .. The 119 nt acceptor RNA template were generated from T7 RNA polymerase Megashortscript kit (Ambion) using the previously described plasmid pAM 2 30 .



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